Prof Denis Murphy
Professor of BiotechnologyHealth, Sport & Science
CF37 4BD
Tel ext. 3747
Room: GT8118
Qualifications
BA (Hons). Biology/Biochemistry, with CellBiology:
D.Phil, Plant Biochemistry
About
Career Summary
1974-77 D.Phil. thesis on 'Lipid metabolism in the leaves of higher plants’. Department of Biological Sciences, University of York, UK
1977-80 Fulbright Postdoctoral Scholar at Department of Biochemistry and Biophysics, University of California, Davis. Research on plant lipid biochemistry
1980-84 AFRC Postdoctoral Fellow at Research Institute for Photosynthesis, University of Sheffield. Worked on biosynthesis of lipids and their biophysical interactions with the major protein complexes of photosynthetic membranes
1984-85 Royal Society Postdoctoral Fellow at Research School for Biological Sciences, Australian National University and CSIRO, Canberra, Australia. Worked on the role of lipids and proteins in the molecular organisation of photosynthetic membranes
1985-90 New Blood Lecturer in Plant Molecular Biology and Biochemistry at Department of Biological Sciences, University of Durham, UK. Established oilseeds research group investigating the regulation of storage lipid formation and mobilisation in seeds
1990-00 Head of Brassica & Oilseeds Research Department at the John Innes Institute, Norwich, UK. Established a new multi-disciplinary crop-based research department for Brassica and oilseeds research and biotechnology. Supervised programmes on molecular genetics, metabolomics, biochemistry, biotechnology, and plant breeding. Honorary Reader at University of East Anglia (UEA), Awarded Honorary Professorship in Biological Sciences by UEA in 1998
2000-01 Head of Lipoprotein Research Centre, Norwich. Part-time university lecturer and visiting research professor. Scientific writer and consultant on biotechnology.
2001-now Head of Biotechnology Unit and Head of Biological Research at University of Glamorgan, UK
2003 Awarded Personal Chair in Biotechnology at University of Glamorgan, UK
Duties & Responsibilities
University activities
- Head of Biology Research
- Leader of Biological Sciences UoA for RAE
Membership of Internal Committees
- Member of Faculty Research Programmes Committee (FRPC) for HESAS
- Vice-Chair of FRPC for Business School
- Member of Faculty Research Operational Group (FROG)
- Member of Faculty Research & Scolarship Committee
- Member of university RAE group
- Member of HESAS Professoriate
Teaching
- GM Crops and the Environment (MSc module)
- Communities Agriculture and Biotechnology (level 3)
- Nutrition and Alimentary System (level 2)
- Plant Physiology (level 2)
- Diversity of Life (level 1)
Experience
Personal research expertise in plant biotechnology, molecular & cell biology and biochemistry.
Over one hundred publications in internationally peer reviewed journals
Supervisor of 16 successful PhD students and over 16 postdocs/technicians
Raised over £3 million in competitive grant funding
Active as a University academic and teacher with sixteen years teaching experience at the University of Durham (1985-1990), University of East Anglia, & University of Glamorgan UK
Ten years of senior management and administrative experience in a top ranking international research institute
Membership of UK and overseas grant assessment committees, external scrutiny panels for various institutes and strategy forming bodies, UK Foresight panels, RAE Biological Sciences Panel 2006-2008
Reviewing of numerous international grant proposals and research publications
Senior Advisor & Consultant on biotechnology to government bodies and private companies, e.g., expert witness at House of Lords Select Committee, Malaysian Government (2001-now) & DEFRA, UK.
Extensive communication experience in scientific specialty and with the general public, including schools
Author of numerous academic and popular articles and books on biotechnology, agriculture and their social impacts
Manager of Schools Outreach Program on Biotechnology in Wales (from 2001)
Significant media interactions including both UK and overseas press, radio and television
Research
Research Interests
Role of intracellular lipid-associated proteins in storage & trafficking
Lipid metabolism and its manipulation in medicine & agriculture
Plant biotechnology and its public impact
Physiological roles of stress-related proteins, such as metallothioneins and PMSR
I have successfully supervised 16 PhD students since 1989 and have raised a total of over £3 million in competitive funding since 1985
Selected Publications
Over 240 significant publications, over 120 of which are in international refereed journals
Scientific Publishing
Currently writing new textbook on Plant Biotechnology, CABI press, publication due late 2009
Commissioned and edited Plant Lipids, Biology Utilisation and Manipulation book for Blackwell Scientific Press, 2004
One of three senior editors responsible for commissioning, reviewing and editing a major three-volume reference work, Encyclopedia of Applied Plant Science, published by Elsevier, in 2003.
Commissioned and edited Designer Oil Crops book for VCH Press, 1994.
Recent books
Murphy DJ (2007) Plant Breeding and Biotechnology : Societal Context and the Future of Agriculture, Cambridge University Press, UK, ISBN: 0521823897.
Murphy DJ (2007) People, Plants, and Genes : The Story of Crops and Humanity, Oxford University Press, ISBN-10: 0-19-920714-3.
Murphy DJ (2007) Fats, genes, and food, In: Weslake R, ed., Teaching Innovations in Lipid Science , Taylor & Francis and CRC Press, USA, in press.
Evans M, Harwood JL, Murphy DJ and Ramji DP (2007) Medical and Agricultural Aspects of Lipids, In: Gunstone FD, Harwood JL and Dijkstra AJ eds., The Lipid Handbook, 3rd Edition, Taylor and Francis, UK,
Murphy DJ (2006) Plant breeding to change fatty acid composition, In: Modifying lipids for use in food, Gunstone F ed., pp 273-305, Woodhead Publishing, Cambridge UK
Murphy DJ (2005) Plant Lipids: biology, utilisation and manipulation, Blackwell, Oxford, UK,
Recent papers:
Murphy DJ (2008) Future prospects for Biofuels, Chemistry Today 26, 44-48 http://www.teknoscienze.com/testata.asp?id_testata=123
Murphy DJ (2008) Muslims transformed medieval Mediterranean countries, SciDevNet, January 2008, http://www.scidev.net/en/south-east-asia/opinions/how-muslims-transformed-mediterranean-countries.html
Murphy DJ (2007) Improved containment strategies in biopharming, Plant Biotechnology J. 5, 555-519, http://www.blackwell-synergy.com/doi/abs/10.1111/j.1467-7652.2007.00278.x
Murphy DJ (2007) Future prospects for oil palm in the 21st century: Biological and related challenges, Eur. J. Lipid Sci. Technol. Special issue on oil palm, Published Online: 15 Mar 2007, DOI: 10.1002/ejlt.200600229.
Murphy DJ (2006) Monster, marvel, or just misunderstood? Public Service Review 12, 114-116,
Murphy DJ (2006) Public good or private gain? Science and Public Affairs, September 2006, p27.
Murphy DJ (2006) Molecular breeding strategies for the modification of lipid composition, In Vitro Cellular and Developmental Biology-Plant, 42, 89-99.
Murphy DJ (2006) The extracellular pollen coat in Brassicaceae: Composition, biosynthesis and functions in pollination , Protoplasma, 228, 31-39.
Sundby C, Härndahl U, Gustavsson N, Åhrman E and Murphy DJ (2005) Conserved methionines in chloroplasts , Biochim. Biophys. Acta 1703, 191-202,
Poghosyan Z, Giannoulia K, Katinakis P, Murphy DJ, Hatzopoulos P (2005) Temporal and transient expression of enoyl-ACP reductase gene during flower and fruit development, Plant Physiology and Biochemistry, 43, 37-44,
Murphy DJ (2005) Demystifying genomics and other ‘omic’ technologies, Inform 16, 732-734 http://www.aocs.org/press/inform/index.asp
Bechtold U, Murphy DJ and Mullineaux PM (2004) Peptide methionine sulphoxide reductase 2 prevents oxidative damage of proteins in the dark, Plant Cell 16, 908-919,
Partridge M and Murphy DJ (2004) Detection of genetically modified soya in a range of organic and health food products. Implications for the accurate labelling of foodstuffs derived from potential GM crops. British Food J. 106, 166-180,
Earlier publications
2004
Bechtold U, Murphy DJ and Mullineaux PM (2004) Peptide methionine sulphoxide reductase 2 prevents oxidative damage of proteins in the dark, Plant Cell 16, 908-919
Thomas B, Murphy DJ and Murray B, editors (2003) Encyclopedia of Applied Plant Sciences, Elsevier, Oxford
Partridge M and Murphy DJ (2004) Detection of genetically modified soya in a range of organic and health food products. Implications for the accurate labelling of foodstuffs derived from potential GM crops. British Food J. 106, 166-180
Murphy, DJ (2004) Biotechnology, its impact and future prospects, in Molecular to Global Photosynthesis (Archer, MA and Barber, J eds), Imperial College Press, London http://www.icpress.co.uk/books/lifesci/p218.html
Murphy DJ (2004) Genetic engineering of lipid composition, in Biotechnology in Agriculture and Forestry, Vol 54 Brassica, ed Pua EC and Douglas CJ, Springer, Berlin
Murphy DJ (2004) Overview of applications of Plant Biotechnology, in Handbook of Plant Biotechnology, eds Christou P and Klee H, Wiley, New York
Murphy DJ (2004) Genetically modified oil crops, in Encyclopedia of Plant and Crop Science, ed Goodman RB, Marcel Dekker, New York
2003
Murphy DJ (2003) Working to improve the oil palm crop, Inform 14, 670-671
Todd A and Murphy DJ (2003) ‘Evaluating University Masterclasses and School Visits as mechanisms for enhancing teaching and learning experiences for undergraduates and school pupils’. Bioscience Education Electronic Journal, published online, Nov 2003 http://bio.ltsn.ac.uk/journal/vol2/index.htm
Murphy DJ (2003) Agricultural biotechnology and oil crops – current uncertainties and future potential, in Appl Biotech Food Sci Policy 1, 25-38
Murphy DJ (2003) Novel proteins from plants help fight hepatitis infection, Advances Wales 39, 21
Murphy, DJ (2003) The uncertain future of agbiotech, Malaysian Oil Science and Technology 12, 1-11
2002
Gustavsson N, Kokke BPA, Härndahl U, Silow M, Bechtold U, Poghosyan Z, Murphy DJ, Boelens WC and Sundby C (2002) A peptide methionine sulfoxide reductase highly expressed in photosynthetic tissue in Arabidopsis thaliana can protect the chaperone-like activity of a chloroplast-localized small heat shock protein, Plant Journal 29:5 p. 545-549
Li M, Murphy DJ, Lee KH, Wilson R, Smith LJ, Clark DC, Sung JY. (2002) Purification and structural characterization of the central hydrophobic domain of oleosin. J Biol Chem. 2002 Oct 4; 277(40): 37888-95. http://www.jbc.org/cgi/reprint/277/40/37888
Hope RG, Murphy DJ & McLauchlan J (2002) The domains required to direct core proteins of hepatitis C virus and GB virus-B to lipid droplets share common features with plant oleosin proteins. J Biol Chem. 277, 4261-4270. http://www.jbc.org/cgi/reprint/277/6/4261
Siti Nor Akmar A, Cheah SC, and Murphy DJ (2002) Isolation and characterisation of two divergent type 3 metallothioneins from oil palm, Elaeis guineensis. Plant Physiol. Biochem. 40, 255-263
Murphy DJ (2002) Novel oils from plants – genes, dreams and realities, Phytochemistry Reviews 1, 67-77 http://reo.nii.ac.jp/journal/HtmlIndicate/Contents/SUP0000001000/JOU0001000496/ISS0000008263/ART0000088455/ART0000088455_abstract.html
2001
Murphy DJ (2001) Biogenesis and functions of lipid bodies in animals, plants and microorganisms. Prog Lipid Res. 40, 325-438
Hernandez-Pinzon I, Patel, K & Murphy DJ (2001) The novel calcium-binding protein, caleosin, has distinct endoplasmic reticulum and lipid-body associated isoforms, Plant Physiol Biochem 39, 1-9
Murphy DJ, Hernandez-Pinzon I, Patel K (2001) Roles of lipid bodies and lipid-body proteins in seeds and other tissues J. Plant Physiol. 158, 471-8
Murphy, DJ (2001) New oil crops for the 21st Century. What is in the pipeline and what is coming in the future, in: MOST, 10, 17-21
2000
Sadanandom A, Poghosyan A, Fairbairn DJ, and Murphy DJ (2000) Differential Regulation of Plastidial and Cytosolic Isoforms of Peptide Methionine Sulfoxide Reductase in Arabidopsis Plant Physiol. 123: 255-264, http://www.plantphysiol.org/cgi/reprint/123/1/255.pdf
Naested H, Frandsen GI, Jauh GY, Hernandez-Pinzon I, Nielsen HB, Murphy DJ, Rogers JC, Mundy J. (2000) Caleosins: Ca2+-binding proteins associated with lipid bodies. Plant Mol Biol. 44, 463-76
Giannoulia K, Haralampidis K, Poghosyan Z, Murphy DJ, Hatzopoulos P (2000) Differential expression of diacylglycerol acyltransferase (DGAT) genes in olive tissues. Biochem Soc Trans. 28, 695-7
http://www.biochemsoctrans.org/bst/028/0695/bst0280695.htm
Murphy DJ, Hernandez-Pinzon I, Patel K, Hope RG, McLauchlan J. (2000) New insights into the mechanisms of lipid-body biogenesis in plants and other organisms. Biochem Soc Trans. 28, 710-1. http://www.biochemsoctrans.org/bst/028/0710/bst0280710.htm
Murphy DJ (2000) Biotechnological applications of seed biology. In: Seed Biology Advances and Applications: Proceedings of the Sixth International Workshop on Seeds, Merida, Mexico, 1999 (Black M, Bradford KJ and Vazquez-Ramos J, editors.) CABI Publishing, Wallingford, Oxford, pp 427-438
http://www.abe.com.pl/html/english/details.php?id=0851994040
Ross JHE, Milanesi C, Murphy DJ and Cresti M (2000) Fixation by rapid-freeze substitution improves tissue preservation of microspores and tapetum in Brassica napus. Sex Plant Reprod, 12: 237-240
http://www.springerlink.com/app/home/contribution.asp?wasp=gmjwrwtqqle18clrrvv3&referrer=parent&backto=issue,7,10;journal,25,45;linkingpublicationresults,id:100456,1
Murphy DJ (2000) Development of New Oil Crops in the 21st Century. Inform 11: 112-117
Murphy DJ (2000) Reply to Jones et al Immunol Today. 2000 Mar; 21(3): 155-6
1999
Murphy DJ (1999) Is rapeseed really an allergenic plant? Popular myths versus scientific realities. Immunol Today. 20, 511-514
Hernandez-Pinzon I, Ross JH, Barnes KA, Damant AP, Murphy DJ (1999) Composition and role of tapetal lipid bodies in the biogenesis of the pollen coat of Brassica napus. Planta. 208, 588-98
Martsinkovskaya AI, Poghosyan ZP, Haralampidis K, Murphy DJ, Hatzopoulos P. (1999) Temporal and spatial gene expression of cytochrome B5 during flower and fruit development in olives. Plant Mol Biol. 40, 79-90 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10394947&dopt=Abstract
Murphy DJ. (1999) Manipulation of plant oil composition for the production of valuable chemicals. Progress, problems, and prospects. Adv Exp Med Biol. 464, 21-35. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10335383&dopt=Abstract
Murphy DJ (1999) The future of new and genetically modified oil crops. In: Perspectives on New Crops and New Uses, (ed Jules Janick) Proceedings of the Fourth National Symposium, New Crops and New Uses Biodiversity and Agricultural Sustainability November 1998, Phoenix, Arizona, USA. Published by ASHS Press, Alexandria, VA
http://www.hort.purdue.edu/newcrop/proceedings1999/v4-216.html
Siti Nor Akmar A, Cheah SC, Aminah S, Leslie CLO, Sambanthamurthi R and Murphy DJ (1999) Characterization and regulation of the oil palm (Elaeis guineensis) stearoyl-ACP desaturases genes. J Oil Palm Res. Special issue, Oct 1999, 1-17
Murphy DJ (1999) Improving Plant Oils for Edible and Industrial Use, Nature Biotechnology 17, suppl 40, http://www.nature.com/cgi-taf/DynaPage.taf?file=/nbt/journal/v17/n5s/full/nbt1199supp2_40.html
Murphy DJ (1999) Le colza carbure aux transgènes (Energy production from rapeseed oil.) Biofutur 195:22-23
Murphy DJ & Vance J. (1999) Mechanisms of lipid-body formation. Trends Biochem Sci. 24, 109-15.
Murphy DJ (1999) Production of novel oils in plants. Curr Opinion Biotechnol. 10, 175-80.
Murphy, DJ (1999) Plant Storage Lipids, in Encyclopedia of Life Sciences, Nature Publishing Group, London, www.els.net
Poghosyan Z, Haralampidis K, Martsinkovskaya AI, Murphy DJ & Hatzopoulos P (1999) Developmental regulation and spatial expression of a plastidial fatty acid desaturase from Olea europaea. Plant Physiol. Biochem. 37, 109-119
1998
Murphy, D.J. (1998) Why is rapeseed pollen such a sticky subject? In: Reproductive Biology. The Royal Botanic Gardens, Kew (S.J. Owens and P J Rudall eds) pp465-474, http://www.rbgkew.org.uk/data/new.html
Murphy, D.J. & Piffanelli, P. (1998) Fatty acid desaturases: structure, mechanism and regulation. In: Plant Lipid Biosynthesis: Fundamentals and Agricultural Applications (Harwood, J.L. ed.), Cambridge University Press, pp 95-130
Leprince, O, van Aelst, AC, Pritchard, HW & Murphy, DJ (1998) Oleosins prevent oil-body coalescence during seed imbibition as suggested by a low-temperature scanning electron microscope study of desiccation-tolerant and -sensitive oilseeds, Planta 204, 109-19
http://www.springerlink.com/app/home/contribution.asp?wasp=b5uc16yhlhdvugykklby&referrer=parent&backto=issue,14,16;journal,72,86;linkingpublicationresults,id:100484,1
Murphy DJ (1998) Impact of genomics on improving the quality of agricultural products. In: Genomics: Commercial Opportunities from a Scientific Revolution (Dixon, G.K., Copping, L.C. and Livingstone, D., eds.), SCI, pp 199-210.
http://www.soci.org/SCI/publications/2001/html/pb29.jsp
Murphy, D.J. & Ross, J.H.E. (1998) Biosynthesis, targeting and processing of oleosin-like proteins, which are major pollen coat components in Brassica napus. Plant Journal 13, 1-16.
Piffanelli, P Ross, JHE & Murphy, DJ (1998) Biogenesis and function of the lipidic structures of pollen grains, Sexual Plant Reproduction 11, 65-80, http://www.springerlink.com/app/home/contribution.asp?wasp=088nnvvhql53hdu6ekdw&referrer=parent&backto=issue,1,7;journal,33,45;linkingpublicationresults,id:100456,1
Piffanelli P & Murphy DJ (1998) Lipid accumulation and related gene expression in gametophytic and sporophytic anther tissues. In: Fertilization in higher plants (Cresti M., Cai G., Moscatelli A. eds), Springer Verlag, Heidelberg, pp 23-43, http://www.amazon.co.uk/exec/obidos/ASIN/3540648798/ref=sr_aps_books_1_1/202-6507772-1595824
Piffanelli P & Murphy DJ (1998) Novel organelles and targeting mechanisms in the anther tapetum. Trends in Plant Science 3, 250-253.
Murphy DJ (1998) Plant lipids: their metabolism, function and utilisation. In: Plant Biochemistry and Molecular Biology (Lea, P.J. and Leegood, R.C., eds.), Wiley New York, pp 119-135
http://www.wileyeurope.com/WileyCDA/WileyTitle/productCd-0471976830.html
Watson, M.D. & Murphy DJ (1998) Genome organization, protein synthesis and processing in plants. In: Plant Biochemistry & Molecular Biology, 2nd Edition (Lea, P.J. & Leegood, R.C. eds) Wiley, New York, pp 235-259, http://www.wileyeurope.com/WileyCDA/WileyTitle/productCd-0471976830.html
Poghosyan, Z., Haralampidis, K., Giannoulia, K., Murphy DJ, Hatzopoulos, P. (1998) Developmental regulation and spatial expression of an ?-3 fatty acid desaturase from Olea europaea. In: Advances in Plant Lipid Research (Sanchez J., Cerdá-Olmedo E., and Martinez-Force E., eds.) Universidad de Sevilla, pp 155-158.
Murphy DJ (1998) Mechanisms of lipid body formation, targeting and accumulation in plants and animals. In: Advances in Plant Lipid Research (Sanchez J., Cerdá-Olmedo E., and Martinez-Force E., eds.) Universidad de Sevilla, pp 683-688.
Hernández-Pinzón, I., Ross, J.H.E. & Murphy DJ (1998) Characterisation of fibrillin-like and oleosin-like proteins in anther lipid bodies of Brassica napus. In: Advances in Plant Lipid Research (Sanchez J, Cerdá-Olmedo E, and Martinez-Force E, eds.) Universidad de Sevilla, pp 689-691.
Hatzopoulos P, Poghosyan ZP, Haralampidis K, Martsinkovskaya AI, Giannoulia, K, Murphy DJ (1998) Spatial, temporal and developmental regulation of expression of genes involved in oil biosynthesis during flower and fruit development. In: Advances in Plant Lipid Research (Sanchez J, Cerdá-Olmedo E, and Martinez-Force E, eds.) Universidad de Sevilla, pp 637-640.
1997
Piffanelli P, Ross JHE, Murphy DJ (1997) Intra- and extracellular lipid composition and associated gene expression patterns during pollen development in Brassica napus, Plant Journal 11, Issue 3, 549-562
http://www.blackwell-synergy.com/servlet/useragent?func=synergy&synergyAction=showAbstract&doi=10.1046/j.1365-313x.1997.11030549.x&area=production&prevSearch=allfield%3A%28murphy%29
Sarmiento C, Ross JHE, Herman E, Murphy DJ (1997) Expression and subcellular targeting of a soybean oleosin in transgenic rapeseed. Implications for the mechanism of oil-body formation in seeds, Plant Journal 11, 783-799, http://www.blackwell-synergy.com/servlet/useragent?func=synergy&synergyAction=showAbstract&doi=10.1046/j.1365-313x.1997.11040783.x&area=production&prevSearch=allfield%3A%28murphy%29
Abell BM, Holbrook LA, Abenes M, Murphy DJ, Hills MJ, Moloney MM. (1997) Role of the proline knot motif in oleosin endoplasmic reticulum topology and oil body targeting. Plant Cell. 1997 Aug; 9(8): 1481-93, http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=157013&action=stream&blobtype=pdf
Hong HP, Ross JH, Gerster JL, Rigas S, Datla RS, Hatzopoulos P, Scoles G, Keller W, Murphy DJ, Robert LS. (1997) Promoter sequences from two different Brassica napus tapetal oleosin-like genes direct tapetal expression of beta-glucuronidase in transgenic Brassica plants. Plant Mol Biol. 1997 Jun; 34(3): 549-555, http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9225865&dopt=Abstract
Piffanelli P, Ross JH, Murphy DJ (1997) Intra- and extracellular lipid composition and associated gene expression patterns during pollen development in Brassica napus. Plant J. 1997 Mar; 11(3): 549-562
http://www.blackwell-synergy.com/openurl?genre=article&sid=nlm:pubmed&issn=0960-7412&date=1997&volume=11&issue=3&spage=549
Murphy DJ (1997) Recent progress in the production of novel renewable oils via biotechnology. In: Renewable Bioproducts. Proceedings of the Wageningen Symposium, June 1997, European Commission, Brussels, pp 68-72.
Leprince, O., van Aelst, A.C., Pritchard, H.W., Murphy DJ (1997) The role of oleosins in oil-body stability during drying and imbibition of desiccation-tolerant and sensitive oilseeds: a low temperature scanning electron microscope study. Proceedings of Dutch Society for microscopy. Nederlands Vermeging voor Microcscopie. Yaarbook 1997. November 27-28 1997.
Murphy DJ, Sarmiento, C., Ross, J.H.E. & Herman, E. (1997) Oleosins: their subcellular targeting and role in oil-body ontogeny. In: Physiology, Biochemistry and Molecular Biology of Plants Lipids (Williams, J.P., Khan, M.U. and Lem, N.W., eds.) Kluwer Academic Press, Dordrecht, Netherlands, pp 289-291
1996
Ross JHE & Murphy DJ (1996) Characterization of anther-expressed genes encoding a major class of extracellular oleosin-like proteins in the pollen coat of Brassicaceae, Plant Journal 9, 625-637 http://www.blackwell-synergy.com/servlet/useragent?func=synergy&synergyAction=showAbstract&doi=10.1046/j.1365-313x.1996.9050625.x&area=production&prevSearch=allfield%3A%28murphy%29
Sadanandom A, Piffanelli P, Knott T, Robinson C, Sharpe A, Lydiate D, Murphy DJ & Fairbairn D (1996) Identification of a peptide methionine sulfoxide reductase gene in an oleosin promoter from Brassica napus. Plant Journal 10, 235-242. http://www.blackwell-synergy.com/servlet/useragent?func=synergy&synergyAction=showAbstract&doi=10.1046/j.1365-313x.1996.10020235.x&area=production&prevSearch=allfield%3A%28murphy%29
Murphy DJ (1996) Engineering oil production in rapeseed and other oil crops. Trends in Biotechnol. 14, 206-213. http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TCW-3W259JS-1V-1&_cdi=5181&_orig=search&_coverDate=06%2F30%2F1996&_sk=999859993&view=c&wchp=dGLbVlb-lSzBV&_acct=C000022838&_version=1&_userid=477543&md5=2f7922ee912758960ace542ddca2742a&ie=f.pdf
Murphy DJ (1996) Biodiesel and its prospects. NRC-PBI Bulletin, Saskatoon, Canada May, pp8-10.
Murphy DJ (1996) Use of biotechnology to improve oil crops. Biotechnology in Agriculture, March 1996, London.
Murphy DJ & Ross JHE (1996) A new class of extracellular proteins found in pollen grains. GCIRC Bulletin 12, 45-46
Murphy DJ, Hills, MJ, Sarmiento, C, Leprince O, Lacey D, Ross JHE and van Aelst AC (1996) Mechanisms of storage oil deposition in plants. GCIRC Bulletin 13, 66-68
Siti Nor Akmar, A., Murphy, D.J. and Cheah, S.C. (1996) Isolation of Mesocarp-Specific cDNA Clones from the Oil Palm by Subtractive Hybridization. In Proc. 1996 International Palm Oil Congress, Competitiveness for the 21st Century (Darus A, Wahid, M.B., Rajanaidu N, Dolmat HT, Paranjothy K, Choo CS, Choong CK, Ravigadevi S, eds), Palm Oil Research Institute of Malaysia, pp 552-556.
1995
Murphy DJ (1995) The use of conventional and molecular genetics to produce new diversity in seed oil composition for the use of plant breeders – progress, problems and future prospects. Euphytica 85, 433-440.
The same review was also reprinted in: Developments in Plant Breeding, Vol 3, The Methodology of Plant Genetic Manipulation: Criteria for Decision Making (Cassels, A.C. & Jones, P.W., eds) Kluwer Academic Publishers, Dordrecht, pp 433-440
Murphy DJ (1995) New oils for old. Chemistry in Britain. April 1995, pp 300-302
Murphy DJ & Mithen RF (1995) Biotechnology. In: Production and Utilization of Brassica Oilseeds (Kimber, D. & McGregor, I., eds.) CAB Press, pp 177-193
Carruthers, S.P., Marsh, J.S., Turner, P.W., Ellis, F., Murphy, D.J., Slabas, T. & Chapman, B.A. (1995) Industrial markets for UK produced oilseeds. Centre for Agricultural Strategy, Research Review No 059. University of Reading.
Murphy, D.J. (1995) Designer Oilseed Rape – Biotechnological possibilities and commercial realities. Proceedings of the International GCIRC Rapeseed Congress, Vol. 4, pp 1332-1334, Cambridge, July 1995.
Murphy, D.J. (1995) Novel oils from plants. Science & Public Affairs. Autumn ed. 44-48.
Mithen RF & Murphy DJ (1995) Biotechnology and Rapeseed Improvement. In: (Scarisbrick, DH & Ferguson, AJ, eds.) New Horizons for Oilseed Rape. Semundo Ltd, Cambridge, U.K. pp 31-43.
Murphy DJ, Ross JHE & Pritchard HW (1995) Are oleosins only associated with oil bodies from desiccation tolerant plant tissues? In: Plant Lipid Metabolism (Kader JC & Mazliak P, eds.) Kluwer Academic Publishers, Dordrecht. pp 558-560.
1994
S. P. Slocombe, P. Piffanelli, D. Fairbairn, S. Bowra, P. Hatzopoulos, M. Tsiantis, and DJ Murphy (1994) Temporal and Tissue-Specific Regulation of a Brassica napus Stearoyl-Acyl Carrier Protein Desaturase Gene Plant Physiol. 104: 1167-1176 http://www.plantphysiol.org/cgi/reprint/104/4/1167.pdf
Murphy DJ. (1994) Manipulation of lipid metabolism in transgenic plants: biotechnological goals and biochemical realities. Biochem Soc Trans. 22, 926-31.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=7698486&dopt=Abstract
Murphy DJ. (1994) Biogenesis, function, and biotechnology of plant storage lipids. Prog Lipid Res. 33, 71-85
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8190744&dopt=Abstract
Keddie JS, Tsiantis M, Piffanelli P, Cella R, Hatzopoulos P, Murphy DJ (1994) A seed-specific Brassica napus oleosin promoter interacts with a G-box-specific protein and may be bi-directional. Plant Mol Biol. 24, 327-40
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8111035&dopt=Abstract
Batchelder, C., Ross, J.H.E. & Murphy DJ (1994) Synthesis and targeting of Brassica napus oleosin in transgenic tobacco. Plant Sci. 104, 39-47
Cummins, I., Hills, M.J., Ross, J.H.E., Hobbs, D.H., Watson, M.D. & Murphy, D.J. (1994) Differential, temporal and spatial expression of genes involved in storage oil and oleosin accumulation in developing rapeseed embryos: implications for the role of oleosins and the mechanisms of oil-body formation. Plant Mol. Biol. 23, 1015-1027.
Murphy DJ (1994) Genetically engineered plant oils. Biol. Sci. Rev. 6, 16-19
Murphy DJ (1994) Biotechnology of oil crops. In: Designer Oil Crops – (Murphy, DJ, ed) VCH Press, Weinheim, pp 219-251
Murphy DJ (1994) Future perspectives for the improvement of oil crops. Designer Oil Crops – (Murphy, D.J. ed) VCH Press. Weinheim, Germany, pp 297-310
Murphy DJ, Richards, R., Taylor, R.D., Capdevielle, J., Guillemot, J.-C., Grison, R., Fairbairn, D. & Bowra, S. (1994) Manipulation of seed oil content to produce industrial crops. Industrial Crops & Products 3, 17-27
Murphy DJ (1994) Transgenic plants – a future source of novel edible and industrial oils, Lipid Technology 6, 84-91
Mithen, R.F. & Murphy, D.J. (1994) Application of biotechnology to the improvement of Brassica oilseed crops. Aspects of Applied Biology. 39, 23-34
1993
Michael R Roberts, Rachel Hodge, Jo H.E Ross, Anna Sorensen, Murphy DJ, John Draper, Rod Scott (1993) Characterization of a new class of oleosins suggests a male gametophyte-specific lipid storage pathway, Plant Journal 3, 629-636 http://www.blackwell-synergy.com/servlet/useragent?func=synergy&synergyAction=showAbstract&doi=10.1046/j.1365-313x.1993.03050629.x&area=production&prevSearch=allfield%3A%28murphy%29
Cummins I, Hills MJ, Ross JH, Hobbs DH, Watson MD, Murphy DJ (1993) Differential, temporal and spatial expression of genes involved in storage oil and oleosin accumulation in developing rapeseed embryos: implications for the role of oleosins and the mechanisms of oil-body formation. Plant Mol Biol. 23, 1015-27
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8260622&dopt=Abstract
Li M, Keddie JS, Smith LJ, Clark DC, Murphy DJ (1993) Expression and characterization of the N-terminal domain of an oleosin protein from sunflower. J Biol Chem. 268, 17504-17512.
http://www.jbc.org/cgi/reprint/268/23/17504
Murphy DJ (1993) Structure, function and biogenesis of storage lipid bodies and oleosins in plants.
Prog Lipid Res. 32, 247-280.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8140114&dopt=Abstract
Hills MJ, Watson MD, Murphy DJ (1993) Targeting of oleosins to the oil bodies of oilseed rape (Brassica napus L.). Planta. 1993 Jan; 189(1): 24-29.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=7763356&dopt=Abstract
Ross, J.H.E. & Murphy, D.J. (1993) Differential accumulation of storage products in developing seeds and somatic cell cultures of Daucus carota L. Plant Sci. 88, 1-11
Whitfield, H.V., Murphy, D.J. & Hills, M.J. (1993) Subcellular localization of very long chain fatty acid elongases in oilseeds. Phytochem. 32, 255-258
Murphy, D.J. (1993) Plant Lipids – their metabolism, function and utilisation. In: Plant Biochemistry and Molecular Biology (Lea, P.J. & Legood, R.C., eds), Open University Press, pp 113-128
Murphy, D.J. & Watson, M.D. (1993) Genome Organisation and Protein Processing in Plants. In: Plant Biochemistry and Molecular Biology, (Lea, P.J. & Legood, R.C., eds), Open University Press, pp 197-221
Richards, D.E., Taylor, R.D. and Murphy, D.J. (1993) Localization and possible substrate specificity of the oleate hydroxylase of developing castor bean seeds. Plant Physiol Biochem. 31, 89-94
Murphy, D.J. (1993) Identification and characterisation of genes and enzymes for the production of oils for the oleochemical industry: a review. Industrial Crops and Products, 2, 251-259
Murphy, D.J., Rawsthorne, S. & Hills, M.J. (1993) Storage lipid formation in seeds. Seed Sci. 3, 79-95.
Murphy, D.J. (1993) Designer Oilseeds for Industry. The Agronomist. 2, 6-8
Murphy, D.J. (1993) Biochemical and molecular regulation of storage product formation in oilseeds. in: Biochemistry and Molecular Biology of Membrane and Storage Lipids of Plants (N. Murata and C.R. Somerville, eds.) The American Society of Plant Physiology, USA, pp.228-237.
Murphy DJ (1993) Structure, function, biochemistry and molecular biology of oleosins in oil plants. Inform 4, 922-932
Murphy, D.J. (1993) Brassica & Oilseeds Research in Norwich. Communications from the Cambridge Laboratory, John Innes Centre, Norwich. GCIRC Bulletin 9, 61-69
Ross JHE, Sanchez J., Millan F & Murphy DJ (1993) Differential presence of oleosins in oleogenic seed and mesocarp tissues in olive (Olea europeae) and avocado (Persea americana). Plant Sci. 93, 203-210
Murphy DJ (1993) The biotechnological utilisation of oilseeds. Acta bot. Gallica 140, 767-777
1992
Slocombe SP, Cummins I, Jarvis RP, Murphy DJ. (1992) Nucleotide sequence and temporal regulation of a seed-specific Brassica napus cDNA encoding a stearoyl-acyl carrier protein (ACP) desaturase. Plant Mol Biol. 1992 Oct; 20(1): 151-155.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1515603&dopt=Abstract
Keddie JS, Edwards EW, Gibbons T, Shaw CH, Murphy DJ. (1992) Sequence of an oleosin cDNA from Brassica napus. Plant Mol Biol. 19, 1079-1083
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1511134&dopt=Abstract
Cummins I, Murphy DJ. (1992) cDNA sequence of a sunflower oleosin and transcript tissue specificity. Plant Mol Biol. 1992 Aug; 19(5): 873-876
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1643288&dopt=Abstract
Keddie JS, Hubner G, Slocombe SP, Jarvis RP, Cummins I, Edwards EW, Shaw CH, Murphy DJ. (1992)
Cloning and characterisation of an oleosin gene from Brassica napus. Plant Mol Biol. 1992 Jun; 19(3): 443-453
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1377966&dopt=Abstract
Li M, Smith LJ, Clark DC, Wilson R, Murphy DJ (1992) Secondary structures of a new class of lipid body proteins from oilseeds. J Biol Chem. 267, 8245-8253, http://www.jbc.org/cgi/reprint/267/12/8245
Ross, J.H.E. & Murphy, D.J. (1992) Biosynthesis and localisation of storage proteins, oleosins and lipids during embryo development in Coriandrum sativum and other Umbelliferae. Plant Science. 86, 59-70
Murphy, D.J. (1992) Modifying oilseed crops for non-edible products. Trends in Biotechnol. 10, 84-87
Murphy, D.J. (1992) Seed oils as renewable resources for food and industry. in: Towards Sustainable Crop Production Systems, Monograph Series II, pp73-76. Royal Agricultural Society of England
Murphy, D.J. et al (1992) Brassica & Oilseeds Research GCIRC Bulletin, Paris pp 14-28
Murphy, D.J. (1992) Using biotechnology to create novel crops for industrial applications. UEA Research Newsletter
Murphy, D.J., Ross, J.H.E. & Li Ming. (1992) Structure & differential accumulation of oleosins and other storage products in oilseeds. in: Metabolism, Structure and Utilisation of Plant Lipids (Cherif, A., ed) pp 405-408
Ross, J. & Murphy, D.J. (1992) Ultrastructural & immunological studies on storage product accumulation in olive seed. in: Metabolism, Structure and Utilisation of Plant Lipids (Cherif, A., ed) pp 444-447
Hills, M.J., Watson, M.W. & Murphy, D.J. (1992) In vitro studies of the synthesis of rape (Brassica napus L.) oleosins (oil body proteins) in: Metabolism, Structure and Utilisation of Plant Lipids (Cherif, A., ed) pp 409-412
Richards, D.E., Taylor, R. & Murphy, D.J. (1992) Substrate requirement & subcellular localization of the oleate hydroxylase of developing castor bean seeds. in: Metabolism, Structure and Utilisation of Plant Lipids (Cherif, A., ed) pp 189-192
Slocombe, S.P. & Murphy, D.J. (1992) Investigation of temporal & tissue-specific regulation of the stearoyl-acyl carrier protein (ACP) desaturase in oilseed rape. in: Metabolism, Structure and Utilisation of Plant Lipids (Cherif, A., ed) pp 436-439
Fairbairn, D., Ross, J.H.E., Bowra, S. & Murphy, D.J. (1992) Molecular & biochemical studies of the biosynthesis of storage oil & petroselinic acid in embryos & cell cultures of Umbelliferae. in: Metabolism, Structure and Utilisation of Plant Lipids (Cherif, A., ed) pp 67-70
1991
Murphy DJ, Keen JN, O’Sullivan JN, Au DM, Edwards EW, Jackson PJ, Cummins I, Gibbons T, Shaw CH, Ryan AJ. (1991) A class of amphipathic proteins associated with lipid storage bodies in plants. Possible similarities with animal serum apolipoproteins. Biochim Biophys Acta. 1991 Jan 17; 1088(1): 86-94.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1989697&dopt=Abstract
Hills MJ, Murphy DJ. (1991) Biotechnology of oilseeds. Biotechnol Genet Eng Rev. 1991; 9:1-46.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1797012&dopt=Abstract
Murphy, D.J., Cummins, I., Edwards, E.W., Hills, M.J. & Li, M. (1991) Oleosins -a new class of abundant seed protein in rapeseed. In: Rapeseeds in a Changing World, 8th International GCIRC Congress, Canada, 3, 922-927.
Murphy, D.J. (1991) Genetic engineering of oilseed rape. Central Office of Information Newsletter
Murphy DJ (1991) Designer Oilseed Crops. Genetic engineering of new oilseed crops for edible and non-edible applications. Agro-Industry Hi-Tech. 5, 5-10.
1990
Li G, Knowles PF, Murphy DJ, Marsh D. (1990) Lipid-protein interactions in thylakoid membranes of chilling-resistant and -sensitive plants studied by spin label electron spin resonance spectroscopy. J Biol Chem. 1990 Oct 5; 265(28): 16867-16872. http://www.jbc.org/cgi/reprint/265/28/16867
Li G, Knowles PF, Murphy DJ, Marsh D. (1990) Lipid-protein interactions in stacked and destacked thylakoid membranes and the influence of phosphorylation and illumination. Spin label ESR studies. Biochim Biophys Acta. 1990 May 24;1024(2):278-284.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=2162205&dopt=Abstract
Murphy DJ. (1990) Storage lipid bodies in plants and other organisms. Prog Lipid Res. 1990; 29(4): 299-324.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=2135719&dopt=Abstract
Fehling, E., Murphy, D.J. & Mukherjee, K.D. (1990) Biosynthesis of triacylglycerols containing very long chain acyl moieties in seeds. Plant Physiol. 94, 492-498
Hills MJ & Murphy DJ (1990) The Biotechnology of Oilseeds. Biotechnol. Genetic Eng. Rev. 9, 1-46
Slocombe, S.P. & Murphy, D.J. (1990) Biochemical and Molecular Biological Studies of the Electron Transport Components of Plant Fatty Acid Desaturases. In: Plant Lipid Biochemistry, Structure and Function (Harwood, J.L. & Quinn, P.J., eds). Portland Press, London. pp 134-137
Fawcett, T, Whitfield,H, Edwards,EW & Murphy,DJ (1990) Biochemical & molecular biological characterisation of acyltransferases, In: Plant Lipid Biochemistry, Structure and Function (Harwood, J.L. & Quinn, P.J., eds) Portland Press, London, pp 172-174
Fehling, E., Murphy, D.J. & Mukherjee, K.D. (1990) Biosynthesis of very long chain triacylglycerols in cruciferous oilseeds. In: Plant Lipid Biochemistry, Structure and Function (Harwood, J.L. & Quinn, P.J., eds). Portland Press, London. pp 201-203
Whitfield, H. & Murphy DJ (1990) Storage Lipid Synthesis in Nasturtium (Tropaeolum Majus). In: Plant Lipid Biochemistry, Structure and Function (Harwood, J.L. & Quinn, P.J., eds). Portland Press, London. pp 225-227
Cummins I & Murphy DJ (1990) Mechanism of Oil Body Synthesis and Maturation in Developing Seeds. In: Plant Lipid Biochemistry, Structure and Function (Harwood JL & Quinn PJ, eds) Portland Press, London. pp 231-233.
O’Sullivan, J.N., Hills, M.J. & Murphy DJ (1990) Purification and Properties of Lipase from Oilseed Rape (Brassica napus L.). In: Plant Lipid Biochemistry, Structure and Function (Harwood, J.L. & Quinn, P.J., eds). Portland Press, London. pp 313-315
Murphy DJ (1990) Structure and function of oleosins (oil body proteins) from oilseeds. In: Plant Lipid Biochemistry, Structure & Function (Harwood, J.L. & Quinn, P,J,. eds). Portland Press, London. pp 477-479
Eldborough, KE, Keddie, JS, Murphy, DJ. & Shaw, CH (1990) The identification and characterisation of proteins involved in the transcription of an embryo specific gene encoding the storage protein cruciferin from Brassica napus (oilseed rape) J. Cell Biochem. 15A, 94, A.421
1989
Au DM, Kang AS, Murphy DJ (1989) Low-resolution epitope characterisation in a family of seed apolipoproteins using polyclonal antibodies. Biochim Biophys Acta. 1989 Sep 11; 1005(1): 97-102.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=2476182&dopt=Abstract
Murphy DJ Cummins I, Kang AS. (1989) Synthesis of the major oil-body membrane protein in developing rapeseed (Brassica napus) embryos. Integration with storage-lipid and storage-protein synthesis and implications for the mechanism of oil-body formation. Biochem J. 1989 Feb 15; 258(1): 285-293.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=2930514&dopt=Abstract
Li G, Knowles PF, Murphy DJ, Nishida I, Marsh D. (1989) Spin-label ESR studies of lipid-protein interactions in thylakoid membranes. Biochemistry. 1989 Sep 5; 28(18): 7446-52.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=2554971&dopt=Abstract
Li, G., Horvath, L., Knowles, P.F., Murphy, D.J. & Marsh, D. (1989) Spin label saturation transfer ESR studies of lipid-protein interactions in Photosystem-II-enriched membranes. Biochim. Biophys. Acta, 987, 187-192
Murphy, D.J., Cummins, I. & Kang, A.S. (1989) Immunological investigation of lipases in germinating oilseed rape Brassica napus, J. Sci. Food Agric. 46, 21-31
Murphy DJ & Cummins I (1989) Purification and immunogold localisation of the major oil-body membrane protein of oilseed rape. Plant Sci. 60, 47-54
Murphy DJ, Cummins I & Kang AS (1989) Immunocytochemical and biochemical studies of the mobilisation of oil-bodies and proteins in germinating cotyledons of oilseed rape, Brassica napus L. J. Sci. Food Agric, 48, 209-23
Murphy, D.J. & Cummins, I. (1989) Biosynthesis of seed storage products during embryogenesis in rapeseed Brassica napus. J. Plant Physiol, 135, 63-69
Murphy, D.J., Cummins, I. & Ryan, A.J. (1989) Immunocytochemcial and biochemical study of the biosynthesis and mobilisation of the major seed storage proteins of Brassica napus. Plant Physiol. Biochem., 27, 647-657
Murphy, DJ & Cummins, I (1989) Seed oil-bodies. Their isolation, composition and the role of oil-body lipoproteins. Phytochem. 28, 2063-2069
Murphy, D.J. & Mukherjee, K.D. (1989) Elongases synthesising very long chain monounsaturated fatty acids in developing oilseeds and their solubilisation. Z. Naturforschung, 44c, 629-634
Hills, M.J., Murphy, D.J. & Beevers, H. (1989) Inhibition of neutral lipase from castor bean lipid bodies by Coenzyme A and oleoyl-CoA. Plant Physiol. 89, 1006-1010
Murphy DJ (1989) Oil storage proteins in the Cruciferae and other oilseeds. Cruciferae 14, 116-117
Murphy, D.J., Li, G., Knowles, P.F. & Nishida, I. (1989) The role of lipid-protein interactions in the structure and function of photosynthetic membranes. In: Photosynthesis: Molecular Biology and Bioenergetics, Springer-Verlag, Berlin, pp.399-409
Murphy, D.J. & Mukherjee, K.D. (1989) Biosynthesis of triacylglycerols containing very long chain acyl moieties in seeds. In: The Biological Role of Plant Lipids (Biacs, P.A. ed.) Plenum, New York, 143-146
Murphy, D.J., Li, G., Nishida, I. & Knowles, P.F. (1989) ESR studies of lipid protein interactions in photosynthetic membranes. In: The Biological Role of Plant Lipids (Biacs, P.A. ed.) Plenum, New York, 207-208
Murphy, D.J. & Cummins, I. (1989) Mobilisation of storage lipids in germinating oilseeds, In: The Biological Role of Plant Lipids (Biacs, P.A., Gruiz, K & Kremmer, T. eds.) Plenum, New York, 37-40
Hills M.J. & Murphy, D.J. (1989) Lipase inhibition by CoA and oleoyl-CoA In: The Biological Role of Plant Lipids (Biacs, P.A., Gruiz, K. & Kremmer, T. eds.). Plenum, New York
Murphy, D.J., O’Sullivan, J.N. & Cummins, I. (1989) The ontogeny of oil storage bodies during seed development in Crucifers, In: The Biological Role of Plant Lipids (Biacs, P.A., Gruiz, K. & Kremmer, T. eds.). Plenum, New York, Budapest, 139-142
Murphy, D.J. & Au, D.M.Y. (1989) A new class of highly abundant apolipoproteins involved in lipid storage in oilseeds. Biochem. Soc. Trans. 17, 685-686
Murphy, D.J. (1989) Are the promoter regions of seed storage protein genes suitable for the expression of genes involved in lipid metabolism? Biochem. Soc. Trans. 17, 685-686
Edwards, E.-W. & Murphy, D.J. (1989) Partial purification and properties of a microsomal lyso-PA acyltransferase from oilseed rape. Biochem. Soc. Trans. 17, 684-685
Murphy, D.J., Ryan, A.J. & Shaw, C.H. (1989) Molecular biological and biochemical studies of the regulation of storage product formation in rapeseed. In: (Engineered Storage Products for the Agro Industry (Nijkamp, K. ed) Dechema, Frankfurt), pp. 35-38
Fehling, E., Murphy, D.J. & Mukherjee, K.D. (1989) Isolation and characterisation of key enzymes catalysing the synthesis of very long chain monounsaturated fatty acids in unconventional oilseeds. In: Engineered Storage Products for the Agro Industry (Nijkamp, K. ed) Dechema, Frankfurt, pp. 39-41
1988
Murphy DJ (1988) A highly active soluble diacylglycerol synthesizing system from developing rapeseed, Brassica napus L. Lipids. 1988 Mar; 23(3): 157-63.
Hills MJ, Murphy DJ. (1988) Characterization of lipases from the lipid bodies and microsomal membranes of erucic acid-free oilseed-rape (Brassica napus) cotyledons. Biochem J. 1988 Feb 1; 249(3): 687-93.
Murphy DJ & Mukherjee KD (1988) Biosynthesis of very long chain monounsaturated fatty acids by subcellular fractions of developing seeds, FEBS Lett. 230, 101-104
Murphy, D.J. & Mukherjee, K.D. (1988) Acyltransferases in developing rapeseed (Decker, K.,ed.), Hoppe-Seylers Biological Chemistry 369, 13-14
Murphy, D.J. & Hills, M.J. (1988) Immunological characterisation of lipases from a wide range of oilseed species, In: Biotechnology for the Fats & Oils Industry (Applewhite, T.E. ed.) Amer. Oil. Chem. Soc., Champaign, IL, 335-338
Murphy, D.J. & Cummins I. (1988) Oil body formation in developing rapeseed, Brassica napus, In: Biotechnology for the Fats & Oils Industry (Applewhite, T.E., ed.) Amer. Oil Chem. Soc., Champaign, IL, 310-313
Murphy DJ (1988) Biochemical, molecular biological and biotechnological studies on oilseeds, Cruciferae 13, 5-7
Murphy, D.J. (1988) Mechanism of oil-body formation during embryogenesis in oilseeds, In: Plant Lipids: Targets for Manipulation (Pinfield, N.J., ed.) Chemical Society, London, p.109
1987
Murphy, D.J. and Mukherjee, K.D. (1987) Acyltransferase in subcellular fractions of developing seeds of rape (Brassica napus L.) Lipids, 22, 293-298
Murphy, D.J. (1987) Mechanisms of Lipid-Protein in Photosynthetic Membranes. In: Structure & Function of Plant Lipids (P.K. Stumpf, ed.) Plenum, New York, 189-191
Murphy, D.J. (1987) Acyltransferase in developing seeds of oilseed rape. In: Structure & Function of Plant Lipids (P.K. Stumpf, ed). Plenum, New York, 537-539
1986
Murphy, D.J. (1986) The molecular organisation of the photosynthetic membranes of higher plants. Biochim. Biophys, Acta Reviews on Biomembranes, 864, 33-94
Murphy, D.J. (1986) Reconstitution of energy transfer and electron transport between solubilised pigment-protein complexes of thylakoid membranes. The role of acyl lipids. Photosynthesis Res., 8, 219-233
Murphy, D.J. (1986) The role of lipids and proteins in the structure and function of photosynthetic membranes. In: Current Topics in Lipid Research (R.Klein & B. Schmidt, eds) Royal Soc. Chem. London pp 276-286.
Murphy, D.J. (1986) Structural properties and molecular organisation of the acyl lipids of photosynthetic membranes. Encyclopedia of Plant Physiology Vol. 19, pp 713-725 Photosynthesis 4 Photosynthetic Membranes 5, Springer-Verlag, Berlin and New York
Murphy, D.J. (1986) Lipid-protein interactions in photosynthetic membranes, Biochem. Soc. Trans., 14, 785-786
Murphy, D.J. (1986) The role of lipids and proteins in the structure and function of photosynthetic membranes. In: Current Topics in Lipid Research (R.Klein & B. Schmidt, eds) Royal Soc. Chem. London pp 276-286
1985
Murphy, D.J., Woodrow, I.E. and Mukherjee, K.D. (1985) Substrate specificities of enzymes of the oleate desaturase system from photosynthetic tissue, Biochem. J., 225, 267-270
Murphy, D.J. and Prinsley, R.T. (1985) Interaction of Triton X-100 with the pigment-protein complexes of photosynthetic membranes. Biochem. J. 229, 31-37
Woodrow, I.E., Furbank, R.T., Brooks A., and Murphy, D.J. (1985) The requirements for a steady-state in the C3 reductive pentose phosphate pathway of photosynthesis. Biochim. Biophys. Acta. 807, 263-271
Murphy, D.J., Harwood, J.L., Lee, K.E., Roberto, F. and Stumpf, P.K., (1985) Differential responses of a range of photosynthetic tissues to a substituted pyridazinone, Sandoz 9785. Specific effects on fatty acid desaturation. Phytochemistry, 24, 1923-1929
1984
Woodrow, I.E., Murphy, D.J. and Latzko, E. (1984) Regulation of stromal sedoheptulose 1,7-Bisphosphatase activity by pH and Mg2+ concentration. J.Biol. Chem. 259, 3791-3795
Murphy DJ, Crowther D & Woodrow IE (1984) Reconstitution of light-harvesting chlorophyll-protein complexes with Photosystem II complexes in soybean phosphatidylcholine liposomes. FEBS Lett. 165, 151-155
Murphy, D.J., Mukherjee, K.D. and Latzko, E. (1984) Oleate metabolism in microsomes from developing leaves of Pisum sativum L. Planta, 161, 249-254
Murphy, D.J., Mukherjee, K.D. and Latzko, E. (1984) Solubilization, purification and kinetic properties of three membrane-bound long-chain acyl-coenzyme-A thioesterases from microsomes of photosynthetic tissue. Eur. J. Biochem. 142, 43-48
Murphy, D.J., Mukherjee, K.D. and Woodrow, I.E. (1984) Functional association of a lysophosphatidylcholine acyltransferase and the oleoyl-glycerophosphocholine desaturase in microsomes from developing leaves. Eur. J. Biochem. 139, 373-379
Murphy, D.J. and Woodrow, I.E. (1984) The effects of Triton X-100 and n-octyl ?-D glucopyranoside on energy transfer in photosynthetic membranes, Biochem. J. 224, 989-993
Murphy, D.J. and Woodrow, I.E. (1984) The role of acyl lipids in the function and molecular organisation of photosynthetic membranes. In: 6th Int. Congr. on Photosynthesis, Vol III, pp. 111-114, (Sybesma, E. ed.), Dr Junk, The Hague
Woodrow, I.E., Latzko, E. and Murphy, D.J. (1984) Regulation of stromal sedoheptulose bisphosphatase activity. In: 6th In. Congr. on Photosynthesis, Vol III, pp. 593-596 (Sybesma, E.ed.), Dr Junk, The Hague
Murphy, D.J., (1984) The role of acyl lipids in the reconstitution of energy transfer between pigment-protein complexes of photosynthetic membranes. In: Structure, Function and Metabolism of Plant Lipids (Siegenthaler, P.A. ed.), Elsevier/North Holland, Amsterdam
Murphy, DJ and Knowles, PF (1984) Phospholipid-protein interactions in photosynthetic membranes. An electron spin-resonance study. In: Structure, Function and Metabolism of Plant Lipids (Siegenthaler, PA ed.), Elsevier, Amsterdam, pp. 425-428
Murphy, D.J., Latzko, E., Woodrow, I.E. and Mukherjee, K.D. (1984) The oleate desaturase system of pea leaf microsomes. In: Structure, Function and Metabolism of Plant Lipids, pp. 45-50 (Siegenthaler, P.A. ed.) Elsevier/ North Holland, Amsterdam
1983
Woodrow, I.E., Murphy, D.J. and Walker, D.A. (1983) Regulation of photosynthetic carbon metabolism. The effect of inorganic phosphate on stromal sedoheptulose 1,7-bisphosphatase. Eur. J. Biochem. 132, 121-123
Murphy, D.J., Mukherjee, K.D. and Latzko, E. (1983) Lipid metabolism in microsomal fractions from photosynthetic tissue. Effects of catalase and hydrogen peroxide on oleate desaturation. Biochem. J. 213, 249-252
Murphy, D.J. and Woodrow, I.E. (1983) Lateral heterogeneity in the distribution of acyl lipids and proteins in photosynthetic membranes. Biochim. Biophys. Acta 725, 104-112
Murphy DJ, Woodrow IE, Mukherjee KD & Latzko E (1983) Solubilisation of oleoyl-CoA thioesterase, oleoyl-CoA phosphatidylcholine acyltransferase and oleolyphosphatidylcholine desaturase. FEBS Lett. 162, 442-446.
Murphy, D.J. and Woodrow, I.E. (1983) The lateral segregation model. A new paradigm for the dynamic role of acyl lipids in the molecular organisation of photosynthetic membranes. In “Biosynthesis and function of plant Lipids”. (W.W. Thomson, ed.), pp. 104-125. American Society of Plant Physiologists, Rockville, MD, U.S.A
1982
Murphy, DJ & Walker, DA (1982) Acetyl coenzyme A biosynthesis in the chloroplast. What is the physiological precursor? Planta 156, 84-88
Murphy, DJ & Walker, DA (1982) The properties of transketolase from photosynthetic tissue. Planta 155, 316-320
Murphy DJ (1982) The importance of non-planar bilayer regions in photosynthetic membranes and their stabilisation by galactolipids. FEBS Lett. 150, 19-26
Murphy, D.J. (1982) The biosynthesis of photosynthetic membrane lipid precursors in higher plants and its integration with photosynthetic carbon metabolism. In: “Biochemistry and Metabolism of Plant Lipids”, (Wintermans, J.F.G.M. and Kuiper, P.J.C., eds), pp. 51-56. Elsevier/North Holland, Amsterdam
Stumpf, P.K., Shimakata, T., Eastwell, K., Murphy, D.J., Liedvogel B., Ohlrogge, J.B. and Kuhn, D.N. (1982) Biosynthesis of fatty acids in a leaf cell. In: “Biochemistry and Metabolism of Plant Lipids”, (Wintermans, J.F.G.M. and Kuiper, P.J.C., eds), pp.3-11. Elsevier/North Holland, Amsterdam
1981
Murphy, D.J. and Kuhn, D.N. (1981) The lack of a phospholipid exchange protein activity in soluble fractions of Spinacea oleracea leaves. Biochem. J. 194, 257-264
Murphy DJ and Stumpf PK (1981) The origin of chloroplastic acetyl-CoA. Arch. Biochem. Biophys. 212, 730-739
Murphy DJ & Leech RM (1981) Photosynthesis of lipids from 14CO2 in Spinacea oleracea, Plant Physiol. 68, 762-765
Murphy, DJ & Walker, DA (1981) Aldolase from wheat leaves – its properties and subcellular distribution. FEBS Lett. 134, 163-166
Murphy, D.J. and Stumpf, P.K., (1981) Polyunsaturation systems from higher plants. Methods in Enzymology 72, 768-773
1980
Murphy, D.J. and Stumpf, P.K. (1980) Polyunsaturated fatty acid biosynthesis in cotyledons from germinating and developing Cucumis sativus L. seedlings. Plant Physiol. 66, 660-665
Murphy, D.J. and Stumpf, P.K. (1980) The in vivo pathway of oleate and linoleate desaturation in developing cotyledons of Cucumis sativus L. seedlings. Plant Physiol. 66, 666-671
Harwood, J.L., Roberto, F., Murphy, D.J., Mckeon, T.A., and Stumpf, P.K. (1980) Inhibition of fatty acid biosynthesis by metronidazole. Biochem Soc. Trans. 8, 535-536
Stumpf, P.K., Kuhn, D.N., Murphy, D.J., Pollard, M.R., Mckeon, T., McCarthy, J. (1980) “Oleic Acid, the Central Substrate”. In: Biogenesis and Function of Plant Lipids” pp.3-10, (P.Mazliak, P.Beneviste, C.Costes & R Douce, eds), Elsevier, North Holland
1979
Murphy, D.J. and Stumpf, P.K. (1979) Light-dependent induction of polyunsaturated fatty acid biosynthesis in greening cucumber cotyledons. Plant Physiol. 63, 328-335
Murphy, D.J. and Stumpf, P.K. (1979) Elongation pathway for ?-linolenic acid synthesis in spinach leaves; a re-examination. Plant Physiol. 64, 428-430
Murphy, D.J., Harwood, J.L., St.John, J.B., and Stumpf, P.K. (1979) Effect of a substituted pyridazinone BASF 13-338, on membrane lipid synthesis in photosynthetic tissues. Biochem. Soc. Trans. 8, 119-120
1978
Murphy DJ & Leech RM (1978) The pathway of [14C] bicarbonate incorporation into lipids in isolated photosynthesising spinach chloroplasts, FEBS Lett. 88, 192-196.
1977
Murphy DJ & Leech RM (1977) Lipid biosynthesis from [14C] bicarbonate [2-14C] pyruvate and [1-14C] acetate during photosynthesis by isolated spinach chloroplasts, FEBS Lett. 77, 164-168
1976
Murphy DJ & Leech RM (1976) Co-operative function of chloroplasts in the biosynthesis of small molecules. In: The Intact Chloroplast, pp. 365-401, (J Barber, ed.) Elsevier, North Holland
Professional Memberships
- Biochemical Society
- British Association for the Advancement of Science
- American Association for the Advancement of Science
- Society for Experimental Biology
- American Oil Chemists Society
- Association for Science in Education
External representation
External Scientific Panels and Committees
Membership of a range of panels and committees concerned with grant reviews, scientific strategy and senior staff promotion at both national and international level. Examples include RAE Biological Sciences Panel 2006-2008, Executive Membership of the LINK Programme Management Committee for the Crops for Industrial Use Programme 1990-96 and the Competitive Industrial Materials from Non-Food Crops Programme from 1997-2005. Other committees include MAFF and NFU policy bodies in the UK and internationally bodies such as NSERC in Canada and the Nobel Foundation in the USA. Expert witness at House of Lords Select Committee on non-food crops (May 1999)
Reviewing and refereeing
Peer review referee for:
Science, Nature, Nature Biotechnology, Nature Cell Biology, Nature Methods, Plant Cell, Plant Journal, Journal of Biological Chemistry, Plant Biotechnology Journal, Plant Molecular Biology, Trends in Biochemical Sciences, Trends in Biotechnology, Trends in Plant Science, Immunology Today, EMBO J, Plant Physiology, Planta, European Journal of Biochemistry, Biochemical Journal, Biochimica et Biophysica Acta, Journal Experimental Biology, Plant Cell & Environment, Plant Science, Seed Science Research, Plant Physiology & Biochemistry, Euphytica, Phytochemistry, Agro-Industry Hi-Tech, Journal of Science, Food & Agriculture, Journal of Experimental Botany, Genetics, Theoretical and Applied Genetics, Physiologia Plantarum, Journal of Histochemistry & Cytology
Grant reviewer for:
Leverhume Foundation, Nuffield Foundation, BBSRC, ESRC, DEFRA and DTI (UK), USDA, NSF (USA), NSERC and AVAC (Canada), ISF (Israel), UGC (Hong Kong, China), CNRS (France)
Membership of UK and overseas grant assessment committees, external scrutiny panels for various institutes and strategy forming bodies, UK Foresight panels
Editorial Board membership
Biotechnology Editor of the magazine, INFORM (American Oil Chemists Society)
Public Communication of Science
Biotechnology Outreach Programme
Established in 2002, this programme seeks to inform and educate the wider community, including schools, about the scientific and other issues that underlie modern biotechnology and its application in medicine and agriculture. There is a regular series of Masterclasses in biotechnology for local schools. To date, we have hosted over 30 school groups and hundreds of pupils for day-long visits to the University that include debates on contentious topics like GM crops or human cloning. The programme also includes visits to schools by our students and seminar/debate sessions on biotechnology-related topics. We are evaluating the impact of our activities on the views of pupils by means of questionnaires and statistical analyses for future publication. In January 2003, we launched a new local teacher training initiative with a workshop at the University of Glamorgan on practical techniques for biotechnology education for 15-19 year olds. We have now secured external funding to continue and extend this programme.
Debate on GM crops
We have a high profile in the ongoing debate on GM crops, both locally in Wales and the UK and internationally in Europe/North America and in developing countries, especially in Asia. This involves providing expertise and advice to organisations and government agencies. We also participate regularly in meetings attended by the general public and in media discussions via the press, radio and television.
Examples of public activities in Wales
June 2003, GM public meeting, Swansea, Wales UK
July 2003, GM public meeting, Penarth Cytun Social Justice Committee, Penarth, Wales UK
July 2003, GM crops debate, Friends of the Earth Cymru, Abergavenny, Wales UK
Sept 2003, GM public meeting, Friends of the Earth Cymru, Pontypridd, Wales UK
Oct 2003, GM public meeting, Monmouth, Wales UK
Nov 2003, GM crops debate, Campaign for the Protection of Rural Wales, Brecon, Wales UK
Feb 2004, British Council debate on the scientific basis of GM and organic crops, Pencoed College,
Wales UK
Feb 2004, Schools Masterclass, Genetic Engineering, Monster or Marvel? University of Glamorgan
March 2004, Can we have a GM free Wales? Wrexham Science Festival, Wrexham, Wales UK
March 2004, Schools Masterclass, GM Food, Panacea or Poison? University of Glamorgan
May 2004, Teacher Workshop on Practical Biotechnology for Schools, University of Glamorgan
Invited lectures at national and international meetings 2000 – 04
14th International Plant Lipid Symposium
Cardiff, UK, July2000, Conference organiser, keynote speaker/Chair of session.
8th International Symposium on Plant Seeds
Gatersleben, Germany, August 2000, biotechnology session convenor, invited speaker
Oils and Fats International Congress 2000
Kuala Lumpur, Malaysia, September 2000, invited plenary lecturer
Biotechnology and Non-Food Products
Munster, Germany, October 2000, invited speaker
Oils and Fats International Congress 2000
Kuala Lumpur, Malaysia, September 2000, invited plenary lecturer
Biotechnology and Non-Food Products
Munster, Germany, October 2000, invited speaker
Plant Biotechnology – Better Products from Better Plants
Helsinki, Finland, June 2001, invited speaker
Biotechnology 2002 conference
Virginia Tech University, USA, July 2002, presented verbal paper & poster
International Congress on the Bioscience of Lipids
Graz, Austria, Sept 2002, invited presenter
Oils and Fats International Congress 2002
Kuala Lumpur, Malaysia, October 2002, invited plenary lecturer
Association of Science in Education
Birmingham, UK, Jan 2003, presented paper
Association of Science in Education
Reading UK, Jan 2004, invited speaker
Malaysian Palm Oil Board, Annual Scientific Meeting
Kuala Lumpur, Malaysia, April 2004, invited plenary lecturer
16th International Plant Lipid Symposium
Budapest, Hungary, June 2004, invited speaker
11th International Palynological Congress
Granada, Spain, July 2004, invited speaker
Consultancy
Advisor and consultant to numerous organisations, including political, environmental and agricultural groups, private companies and UK political parties. We offer a range of expert consultancy services in agricultural biotechnology, conventional agriculture and tropical agriculture, especially as relating to oil crops.
Government/Public Bodies
External Scientific Advisor and Chairman of Biology Committee to Malaysian Government Research Programme on Oil Palm at the Malaysian Palm Oil Board,
Programme Advisor and Project Monitor on Oil Crops to UK LINK projects
Previously Advisor to Ministry of Agriculture, Greece; Ministry of Agriculture, Fisheries and Food UK (now DEFRA) and to Canadian National Science and Engineering Council
External examiner (assessment of research programme and staff career development) for universities and research institutes in USA, Malaysia, Israel, Canada
Private Companies and Organisations
Advisor and consultant to numerous private companies and other organisations, including political, environmental and agricultural groups and UK political parties.
We offer a range of expert consultancy services in agricultural biotechnology, conventional agriculture and tropical agriculture, especially as relating to oil crops, including rapeseed, soya, sunflower, oil palm, maize and linseed.
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